PDFRaycheva Ts., Stoyanov K., Naimov S., Apostolova-Kuzova E. 2021.
Crocus adamioides (Iridaceae) in Bulgarian flora
Acta Botanica Croatica, 80(1). (online first)
Crocus adamioides Kernd. et Pasche, as recently known, was originally treated as C. biflorus Mill. subsp. adamii B. Mathew in the flora of Bulgaria by Mathew (1982) and verified by Rukšāns (2017). The taxon was afterwards described as a separate species (Kerndorff et al. 2012), with the holotype collected in Kırklareli Province, European Turkey. The species was for the first time mapped in two floristic regions of Bulgaria. The diagnostic characters based on the general morphology and leaf anatomy were defined from the natural populations of the species and compared to the type specimen and relevant data from the literature. The phylogenetic position of the species was clarified by sequencing of the internal transcribed spacer region (ITS: ITS1 + 5.SsrDNA + ITS2) and comparison of the obtained sequence with those annotated in NCBI. A phylogenetic tree was built using Bayesian phylogeny. Results have shown highest phylogenetic similarity with C. adamioides from Turkey. The closest relative C. ranjeloviciorum Kernd., Pasche, Harpke et Raca remains in proximity. Our morphological, anatomical and molecular analyses have revealed that the Bulgarian population shows a peculiar combination of characters specific to C. adamioides.